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Nom : Nicolas Leulliot

Directeur de l’unité de recherche : Laboratoire de Cristallographie et RMN Biologiques - LCRB - UMR 8015 CNRS - Université Paris Descartes

Professeur Paris Descartes

Membre Junior de l’Institut Universitaire de France (IUF)

Tel : 01-53-73-15-76

Courriel : Nicolas Leulliot

Recherche :

• Protein/RNA structural biology

• Protein-Protein and Protein-RNA complexes

• Proteins involved in ribosome biogenesis pathway

Publications

Robert-Paganin J, Réty S, Leulliot N. Regulation of DEAH/RHA Helicases by G-Patch Proteins. BioMed Research International. 2015 Jan 27 ;2015:e931857.

Loc’h J, Blaud M, Réty S, Lebaron S, Deschamps P, Bareille J, et al. RNA Mimicry by the Fap7 Adenylate Kinase in Ribosome Biogenesis. PLoS Biol. 2014 May 13 ;12(5). Available from : http://www.ncbi.nlm.nih.gov/pmc/art...

Peixeiro N, Keller J, Collinet B, Leulliot N, Campanacci V, Cortez D, et al. Structure and function of AvtR, a novel transcriptional regulator from a hyperthermophilic archaeal lipothrixvirus. J Virol. 2013 Jan ;87(1):124–36.

Leulliot N, Quevillon-Cheruel S, Graille M, Geslin C, Flament D, Le Romancer M, et al. Crystal structure of PAV1-137 : a protein from the virus PAV1 that infects Pyrococcus abyssi. Archaea. 2013 ;2013:568053.

Machado-Pinilla R, Liger D, Leulliot N, Meier UT. Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs. RNA. 2012 Oct ;18(10):1833–45.

Huguet F, Melet A, Alves de Sousa R, Lieutaud A, Chevalier J, Maigre L, et al. Hydroxamic Acids as Potent Inhibitors of FeII and MnIIE. coli Methionine Aminopeptidase : Biological Activities and X-ray Structures of Oxazole Hydroxamate–EcMetAP-Mn Complexes. ChemMedChem. 2012 ;7(6):1020–30.

Walbott H, Machado-Pinilla R, Liger D, Blaud M, Réty S, Grozdanov PN, et al. The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic. Genes & Development. 2011 Nov 15 ;25(22):2398–408.

Collinet B, Friberg A, Brooks MA, van den Elzen T, Henriot V, Dziembowski A, et al. Strategies for the structural analysis of multi-protein complexes : Lessons from the 3D-Repertoire project. Journal of Structural Biology. 2011 Aug ;175(2):147–58.

Walbott H, Mouffok S, Capeyrou R, Lebaron S, Humbert O, van Tilbeurgh H, et al. Prp43p contains a processive helicase structural architecture with a specific regulatory domain. EMBO J. 2010 Jul 7 ;29(13):2194–204.

Leulliot N, Blondeau K, Keller J, Ulryck N, Quevillon-Cheruel S, van Tilbeurgh H. Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD. J Mol Biol. 2010 May 21 ;398(5):641–6.

Goulet A, Redder P, Pina M, Prangishvili D, Vera L, Lichière J, et al. ORF 157 from the Archaeal Virus AFV1 Defines a New Class of Nuclease. J Virol . 2010 Mar 3 ; Available from : http://www.ncbi.nlm.nih.gov.gate1.i...

Quevillon-Cheruel S, Leulliot N, Muniz CA, Vincent M, Gallay J, Argentini M, et al. Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles. J Biol Chem. 2009 Feb 6 ;284(6):3552–62.

Leulliot N, Cladière L, Lecointe F, Durand D, Hübscher U, van Tilbeurgh H. The family X DNA polymerase from Deinococcus radiodurans adopts a non-standard extended conformation. J Biol Chem. 2009 May 1 ;284(18):11992–9.

Keller J, Leulliot N, Collinet B, Campanacci V, Cambillau C, Pranghisvilli D, et al. Crystal structure of AFV1-102, a protein from the acidianus filamentous virus 1. Protein Sci. 2009 Apr ;18(4):845–9.

Keller J, Leulliot N, Soler N, Collinet B, Vincentelli R, Forterre P, et al. A protein encoded by a new family of mobile elements from Euryarchaea exhibits three domains with novel folds. Protein Sci. 2009 Apr ;18(4):850–5.

Goulet A, Spinelli S, Blangy S, van Tilbeurgh H, Leulliot N, Basta T, et al. The thermo- and acido-stable ORF-99 from the archaeal virus AFV1. Protein Sci. 2009 Jun ;18(6):1316–20.

Goulet A, Spinelli S, Blangy S, van Tilbeurgh H, Leulliot N, Basta T, et al. The crystal structure of ORF14 from Sulfolobus islandicus filamentous virus. Proteins. 2009 Sep ;76(4):1020–2.

Godin KS, Walbott H, Leulliot N, van Tilbeurgh H, Varani G. The box H/ACA snoRNP assembly factor Shq1p is a chaperone protein homologous to Hsp90 cochaperones that binds to the Cbf5p enzyme. J Mol Biol. 2009 Jul 10 ;390(2):231–44.

Walbott H, Leulliot N, Grosjean H, Golinelli-Pimpaneau B. The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C5)-methyltransferase provides insights into its tRNA specificity. Nucleic Acids Res. 2008 Sep ;36(15):4929–40.

Leulliot N, Bohnsack MT, Graille M, Tollervey D, Van Tilbeurgh H. The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. Nucleic Acids Res. 2008 Feb ;36(2):629–39.

Leulliot N, Chaillet M, Durand D, Ulryck N, Blondeau K, van Tilbeurgh H. Structure of the yeast tRNA m7G methylation complex. Structure. 2008 ;16(1):52–61.

Quevillon-Cheruel S, Collinet B, Trésaugues L, Minard P, Henckes G, Aufrère R, et al. Cloning, production, and purification of proteins for a medium-scale structural genomics project. Methods in Molecular Biology (Clifton, NJ) . 2007. p. 21–37. Available from : http://www.ncbi.nlm.nih.gov.gate1.i...

Quevillon-Cheruel S, Leulliot N, Gentils L, van Tilbeurgh H, Poupon A. Production and crystallization of protein domains : how useful are disorder predictions  ? Curr Protein Pept Sci. 2007 Apr ;8(2):151–60.

Leulliot N, Godin KS, Hoareau-Aveilla C, Quevillon-Cheruel S, Varani G, Henry Y, et al. The box H/ACA RNP assembly factor Naf1p contains a domain homologous to Gar1p mediating its interaction with Cbf5p. J Mol Biol. 2007 Aug 31 ;371(5):1338–53.

Keller J, Leulliot N, Cambillau C, Campanacci V, Porciero S, Prangishvilli D, et al. Crystal structure of AFV3-109, a highly conserved protein from crenarchaeal viruses. Virol J. 2007 ;4:12.

Hecker A, Leulliot N, Gadelle D, Graille M, Justome A, Dorlet P, et al. An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro. Nucleic Acids Res. 2007 ;35(18):6042–51.

Trésaugues L, Dehé P-M, Guérois R, Rodriguez-Gil A, Varlet I, Salah P, et al. Structural characterization of Set1 RNA recognition motifs and their role in histone H3 lysine 4 methylation. J Mol Biol. 2006 Jun 23 ;359(5):1170–81.

Ramaen O, Leulliot N, Sizun C, Ulryck N, Pamlard O, Lallemand J-Y, et al. Structure of the human multidrug resistance protein 1 nucleotide binding domain 1 bound to Mg2+/ATP reveals a non-productive catalytic site. J Mol Biol. 2006 Jun 16 ;359(4):940–9.

Quevillon-Cheruel S, Leulliot N, Graille M, Blondeau K, Janin J, van Tilbeurgh H. Crystal structure of the yeast His6 enzyme suggests a reaction mechanism. Protein Sci. 2006 Jun ;15(6):1516–21.

Leulliot N, Vicentini G, Jordens J, Quevillon-Cheruel S, Schiltz M, Barford D, et al. Crystal structure of the PP2A phosphatase activator : implications for its PP2A-specific PPIase activity. Mol Cell. 2006 Aug 4 ;23(3):413–24.

Graille M, Baltaze J-P, Leulliot N, Liger D, Quevillon-Cheruel S, van Tilbeurgh H. Structure-based functional annotation : yeast ymr099c codes for a D-hexose-6-phosphate mutarotase. J Biol Chem. 2006 Oct 6 ;281(40):30175–85.

Goulet A, Spinelli S, Campanacci V, Porciero S, Blangy S, Garrett RA, et al. Crystallization and preliminary X-ray diffraction analysis of protein 14 from Sulfolobus islandicus filamentous virus (SIFV). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1 ;62(Pt 9):884–6.

Zhou C-Z, Meyer P, Quevillon-Cheruel S, De La Sierra-Gallay IL, Collinet B, Graille M, et al. Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly-roll fold. Protein Sci. 2005 Jan ;14(1):209–15.

Quevillon-Cheruel S, Leulliot N, Graille M, Hervouet N, Coste F, Benedetti H, et al. Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family. PROTEIN SCIENCE. 2005 May ;14(5):1350–6.

Prilusky J, Oueillet E, Ulryck N, Pajon A, Bernauer J, Krimm I, et al. HalX : an open-source LIMS (Laboratory Information Management System) for small- to large-scale laboratories. Acta Crystallogr D Biol Crystallogr. 2005 Jun ;61(Pt 6):671–8.

Meyer P, Liger D, Leulliot N, Quevillon-Cheruel S, Zhou C-Z, Borel F, et al. Crystal structure and confirmation of the alanine:glyoxylate aminotransferase activity of the YFL030w yeast protein. Biochimie. 2005 Dec ;87(12):1041–7.

Liger D, Quevillon-Cheruel S, Sorel I, Bremang M, Blondeau K, Aboulfath I, et al. Crystal structure of YHI9, the yeast member of the phenazine biosynthesis PhzF enzyme superfamily. Proteins. 2005 Sep 1 ;60(4):778–86.

Leulliot N, Trésaugues L, Bremang M, Sorel I, Ulryck N, Graille M, et al. High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project : one size fits all ? Acta Crystallogr D Biol Crystallogr. 2005 Jun ;61(Pt 6):664–70.

Leulliot N, Quevillon-Cheruel S, Graille M, Schiltz M, Blondeau K, Janin J, et al. Crystal structure of yeast YER010Cp, a knotable member of the RraA protein family. Protein Sci. 2005 Oct ;14(10):2751–8.

Graille M, Meyer P, Leulliot N, Sorel I, Janin J, Van Tilbeurgh H, et al. Crystal structure of the S. cerevisiae D-ribose-5-phosphate isomerase : comparison with the archaeal and bacterial enzymes. Biochimie. 2005 Aug ;87(8):763–9.

Quevillon-Cheruel S, Liger D, Leulliot N, Graille M, Poupon A, de La Sierra-Gallay IL, et al. The Paris-Sud yeast structural genomics pilot-project : from structure to function. Biochimie. 2004 Oct ;86(9-10):617–23.

Quevillon-Cheruel S, Leulliot N, Meyer P, Graille M, Bremang M, Blondeau K, et al. Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiae. J Biol Chem. 2004 Jan 2 ;279(1):619–25.

Liger D, Graille M, Zhou C-Z, Leulliot N, Quevillon-Cheruel S, Blondeau K, et al. Crystal structure and functional characterization of yeast YLR011wp, an enzyme with NAD(P)H-FMN and ferric iron reductase activities. J Biol Chem. 2004 Aug 13 ;279(33):34890–7.

Leulliot N, Quevillon-Cheruel S, Sorel I, Graille M, Meyer P, Liger D, et al. Crystal structure of yeast allantoicase reveals a repeated jelly roll motif. J Biol Chem. 2004 May 28 ;279(22):23447–52.

Leulliot N, Quevillon-Cheruel S, Sorel I, de La Sierra-Gallay IL, Collinet B, Graille M, et al. Structure of protein phosphatase methyltransferase 1 (PPM1), a leucine carboxyl methyltransferase involved in the regulation of protein phosphatase 2A activity. J Biol Chem. 2004 Feb 27 ;279(9):8351–8.

Leulliot N, Quevillon-Cheruel S, Graille M, van Tilbeurgh H, Leeper TC, Godin KS, et al. A new alpha-helical extension promotes RNA binding by the dsRBD of Rnt1p RNAse III. EMBO J. 2004 Jul 7 ;23(13):2468–77.

Graille M, Quevillon-Cheruel S, Leulliot N, Zhou C-Z, de la Sierra Gallay IL, Jacquamet L, et al. Crystal structure of the YDR533c S. cerevisiae protein, a class II member of the Hsp31 family. Structure. 2004 May ;12(5):839–47.

De La Sierra-Gallay IL, Collinet B, Graille M, Quevillon-Cheruel S, Liger D, Minard P, et al. Crystal structure of the YGR205w protein from Saccharomyces cerevisiae : close structural resemblance to E. coli pantothenate kinase. Proteins. 2004 Mar 1 ;54(4):776–83.

Zhou C-Z, de La Sierra-Gallay IL, Quevillon-Cheruel S, Collinet B, Minard P, Blondeau K, et al. Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphate. J Biol Chem. 2003 Dec 12 ;278(50):50371–6.

Quevillon-Cheruel S, Collinet B, Zhou CZ, Minard P, Blondeau K, Henkes G, et al. A structural genomics initiative on yeast proteins. J Synchrotron Radiat. 2003 Jan 1 ;10(Pt 1):4–8.

Quevillon-Cheruel S, van Tilbeurgh H, Leulliot N, Tresaugues L, Liger D, Zhou CZ, et al. The South-Paris Yeast Structural Genomics Project. YEAST. 2003 Jul ;20(Suppl. 1):S340.

Leeper T, Leulliot N, Varani G. The solution structure of an essential stem-loop of human telomerase RNA. Nucleic Acids Res. 2003 May 15 ;31(10):2614–21.

Hernandez B, Baumruk V, Leulliot N, Gouyette C, Huynh-Dinh T, Ghomi M. Thermodynamic and structural features of ultrastable DNA and RNA hairpins. JOURNAL OF MOLECULAR STRUCTURE. 2003 Jun 1 ;651:67–74.

Leulliot N, Varani G. Current topics in RNA-protein recognition : control of specificity and biological function through induced fit and conformational capture. Biochemistry. 2001 Jul 10 ;40(27):7947–56.

Hocquet A, Leulliot N, Ghomi M. Ground-state properties of nucleic acid constituents studied by density functional calculations. 3. Role of sugar puckering and base orientation on the energetics and geometry of 2 `-deoxyribonucleosides and ribonucleosides. JOURNAL OF PHYSICAL CHEMISTRY B. 2000 May 11 ;104(18):4560–8.

Gaigeot MP, Leulliot N, Ghomi M, Jobic H, Coulombeau C, Bouloussa O. Analysis of the structural and vibrational properties of RNA building blocks by means of neutron inelastic scattering and density functional theory calculations. CHEMICAL PHYSICS. 2000 Nov 1 ;261(1-2, Sp. Iss. SI):217–37.

Ulicny J, Leulliot N, Grajcar L, Baron MH, Jobic H, Ghomi M. NIS, IR and Raman spectra with quantum mechanical calculations for analyzing the force field of hypericin model compounds. In : Johnson MR, Kearley GJ, Buttner HG, editors. NEUTRONS AND NUMERICAL METHODS-N(2)M. 1999. p. 183–6.

Leulliot N, Jobic H, Ghomi M. Search for a reliable nucleic acid force field using neutron inelastic scattering and quantum mechanical calculations : Bases, nucleosides and nucleotides. In : Johnson MR, Kearley GJ, Buttner HG, editors. NEUTRONS AND NUMERICAL METHODS-N(2)M. 1999. p. 179–82.

Leulliot N, Ghomi M, Scalmani G, Berthier G. Ground state properties of the nucleic acid constituents studied by density functional calculations. I. Conformational features of ribose, dimethyl phosphate, uridine, cytidine, 5 `-methyl phosphate-uridine, and 3 `-methyl phosphate-uridine. JOURNAL OF PHYSICAL CHEMISTRY A. 1999 Oct 28 ;103(43):8716–24.

Leulliot N, Ghomi M, Jobic H, Bouloussa O, Baumruk V, Coulombeau C. Ground state properties of the nucleic acid constituents studied by density functional calculations. 2. Comparison between calculated and experimental vibrational spectra of uridine and cytidine. JOURNAL OF PHYSICAL CHEMISTRY B. 1999 Dec 9 ;103(49):10934–44.

Leulliot N, Baumruk V, Gouyette C, Huynh-Dinh T, Turpin PY, Ghomi M. Aqueous phase structural features of GNRA tetraloops formed in short hairpins as evidenced by UV absorption and Raman spectroscopy. VIBRATIONAL SPECTROSCOPY. 1999 Apr ;19(2):335–40.

Leulliot N, Baumruk V, du Penhoat CH, Turpin PY, Gouyette C, Huynh-Dinh T, et al. Vibrational spectroscopies, NMR, UV absorption and molecular modeling by DFT calculations to elucidate RNA structures. In : Greve J, Puppels GJ, Otto C, editors. SPECTROSCOPY OF BIOLOGICAL MOLECULES : NEW DIRECTIONS. 1999. p. 207–10.

Leulliot N, Baumruk V, Abdelkafi M, Turpin PY, Namane A, Gouyette C, et al. Unusual nucleotide conformations in GNRA and UNCG type tetraloop hairpins : evidence from Raman markers assignments. Nucleic Acids Res. 1999 Mar 1 ;27(5):1398–404.

Abdelkafi M, Leulliot N, Baumruk V, Bednárová L, Turpin PY, Namane A, et al. Structural features of the UCCG and UGCG tetraloops in very short hairpins as evidenced by optical spectroscopy. Biochemistry. 1998 May 26 ;37(21):7878–84.

Baumruk V, Abdelkafi M, Leulliot N, Bednarova L, Du Penhoat CH, Turpin PY, et al. Common structural features of UNCG tetraloops in very short hairpins determined by UV absorption, Raman, IR and NMR spectroscopies. In : Carmona P, Navarro R, Hernanz A, editors. SPECTROSCOPY OF BIOLOGICAL MOLECULES : MODERN TRENDS. 1997. p. 241–2.

Abdelkafi M, Leulliot N, Ghomi M, duPenhoat CH, Namane A, Gouyette C, et al. UNCG tetraloops in short oligoribonucleotides reveal high thermodynamic stability and unusual structural properties in aqueous phase as confirmed by optical and NMR spectroscopies. JOURNAL OF MOLECULAR STRUCTURE. 1997 Jun 1 ;408:241–5.

Gripp J, Leulliot N, Mielke SL, Orozco LA. Evolution of the vacuum rabi splitting into a single peak for a bistable optical cavity. In : Eberly JH, Mandel L, Wolf E, editors. COHERENCE AND QUANTUM OPTICS VII. 1996. p. 351–2.