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Accueil du site > Annuaire > Isabelle Broutin

Isabelle Broutin

Responsable de l’équipe : Signalisation et transport membranaire

Directrice de Recherche CNRS - Directrice adjointe du laboratoire

Courriel : Isabelle Broutin

Tel : 01 53 73 15 77

Fax : 01 53 73 99 85

- Spécialité :

  • Cristallographie des protéines

- Recherche :

  • Biologie structurale des protéines membranaires
    • Pompes à efflux bactériennes impliquées dans la résistance aux antibiotiques
    • Récepteur au VEGF impliqués dans certains cancers
    • Régulateur d’expression des pompes à efflux de Pseudomonas aeruginosa
  • Mouvements et transitions structurales dans les protéines
  • Cristallogenèse

- Administration de la Recherche :

  • Membre du conseil scientifique de la faculté de pharmacie
  • Membre du conseil scientifique du GEM "Groupe d’Etude des Membranes"
  • Membre du bureau de l’école doctorale "Médicament, Toxicologie, Chimie, Imageries"
  • Membre du conseil de l’école doctorale "genetic and development" UPMC Paris
  • Membre du comité scientifique d’évaluation des demandes d’accès au synchrotron SOLEIL

- Publications des 5 dernières années

- 1. Salvador, D., Glavier M., Schoehn G., Phan G., Taveau JC., Decossas M., Lecomte S., Mongrand S., Garnier C., Broutin I., Daury L., Lambert O. (2019). Minimal nanodisc without exogenous lipids for stabilizing membrane proteins in detergent-free buffer. Biochim Biophys Acta Biomembr, 1861(4), 852-860
- 2. Bonnet, A.L., Chaussain, C., Broutin, I., Rochefort, G.Y., Schrewe, H., and Gaucher, C. (2018). From Vascular Smooth Muscle Cells to Folliculogenesis : What About Vasorin ? Front Med (Lausanne) 5, 335.
- 3. Gomez-Zepeda D, Taghi M, Smirnova M, Sergent P, Liu WQ, Chhuon C, Vidal M, Picard M, Thioulouse E, Broutin I, Guerrera IC, Scherrmann JM, Parmentier Y, Decleves X, Menet MC. (2018). LC-MS/MS-based quantification of efflux transporter proteins at the BBB. J Pharm Biomed Anal., 164:496-508.
- 4. Housseini, B.I.K., Phan, G., and Broutin, I. (2018). Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures. Front Mol Biosci 5, 57.
- 5. Gaviard C, Broutin I, Cosette P, Dé E, Jouenne T, Hardouin J. (2018). Lysine Succinylation and Acetylation in Pseudomonas aeruginosa. J Proteome Res.
- 6. Juarez, P., Broutin, I., Bordi, C., Plesiat, P., and Llanes, C. (2018). Constitutive activation of MexT by amino acid substitutions results in MexEF-OprN overproduction in clinical isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 62(5). pii : e02445-17
- 7. Picard, M., Tikhonova, E.B., Broutin, I., Lu, S., Verchere, A., and Zgurskaya, H.I. (2018). Biochemical Reconstitution and Characterization of Multicomponent Drug Efflux Transporters. Akihito Yamaguchi and Kunihiko Nishino (eds.), Bacterial Multidrug Exporters : Methods and Protocols Methods Mol Biol vol. 1700, 113-145.
- 8. Ntsogo Enguéné YV, Phan G, Garnier C, Ducruix A, Prangé T, Broutin I. (2017) Xenon for tunnelling analysis of the efflux pump component OprN. PLoS One, 12(9):e0184045.
- 9. Verchere A, Broutin I, Picard M (2017) Reconstitution of Membrane Proteins in Liposomes. Methods Mol Biol 1635 : 259-282
- 10. Reille-Seroussi M, Gaucher JF, Cussac LA, Broutin I, Vidal M, Broussy S (2017) VEGFR1 domain 2 covalent labeling with horseradish peroxidase : Development of a displacement assay on VEGF. Anal Biochem 530 : 107-112
- 11. Gomez-Zepeda D, Chaves C, Taghi M, Sergent P, Liu WQ, Chhuon C, Vidal M, Picard M, Thioulouse E, BroNom : Isabelle Broutinutin I, Guerrera IC, Scherrmann JM, Parmentier Y, Decleves X, Menet MC (2017) Targeted unlabeled multiple reaction monitoring analysis of cell markers for the study of sample heterogeneity in isolated rat brain cortical microvessels. J Neurochem 142 : 597-609
- 12. Chaptal V, Delolme F, Kilburg A, Magnard S, Montigny C, Picard M, Prier C, Monticelli L, Bornert O, Agez M, Ravaud S, Orelle C, Wagner R, Jawhari A, Broutin I, Pebay-Peyroula E, Jault JM, Kaback HR, le Maire M, Falson P. (2017) Quantification of Detergents Complexed with Membrane Proteins. Sci Rep., 7:41751.
- 13. Monlezun L., Phan G., Benabdelhak H., Lascombe M.B., Ntsogo Enguéné V.Y., Picard M. and Broutin I. (2015). New OprM structure highlighting the nature of the N-terminal anchor. frontiers research topic "Bad bugs in the XXIst century : resistance mediated by multi-drug efflux pumps in gram-negative bacteria", Edited by : Attilio Vittorio Vargiu, Klaas Martinus Pos, Keith Poole and Hiroshi Nikaido, Published in : Frontiers in Microbiology, pp 60-69
- 14. Ntsogo Enguene, V.Y., Verchère, A., Phan, G., Broutin, I., and Picard, M. (2015). Catch me if you can : a biotinylated proteoliposome affinity assay for the investigation of assembly of the MexA-MexB-OprM efflux pump from Pseudomonas aeruginosa. frontiers research topic "Bad bugs in the XXIst century : resistance mediated by multi-drug efflux pumps in gram-negative bacteria", Edited by : Attilio Vittorio Vargiu, Klaas Martinus Pos, Keith Poole and Hiroshi Nikaido, Published in : Frontiers in Microbiology, pp 113-120
- 15. Gaucher JF, Reille-Seroussi M, Gagey-Eilstein N, Broussy S, Coric P, Seijo B, Lascombe MB, Gautier B, Liu WQ, Huguenot F, Inguimbert N, Bouaziz S, Vidal M, Broutin I. (2016) Biophysical Studies of the Induced Dimerization of Human VEGF Receptor 1 Binding Domain by Divalent Metals Competing with VEGF-A. PLoS One., 11(12):e0167755.
- 16. Daury L, Orange F, Taveau JC, Verchère A, Monlezun L, Gounou C, Marreddy RK, Picard M, Broutin I, Pos KM, Lambert O. (2016) Tripartite assembly of RND multidrug efflux pumps. Nat Commun., 7:10731.
- 17. Bernard V, Bouilly J, Beau I, Broutin I, Chanson P, Young J, Binart N. (2016). Germline Prolactin Receptor Mutation is not a Major Cause of Sporadic Prolactinoma in Humans. Neuroendocrinology, 103(6), 738-45
- 18. Phan G, Picard M, Broutin I. (2015) Focus on the Outer Membrane Factor OprM, the Forgotten Player from Efflux Pumps Assemblies. Antibiotics, 4(4), 544-566
- 19. Berrazeg M, Jeannot K, Ntsogo Enguéné VY, Broutin I, Loeffert S, Fournier D, Plésiat P. (2015). Mutations in ß-Lactamase AmpC Increase Resistance of Pseudomonas aeruginosa Isolates to Antipseudomonal Cephalosporins. Antimicrob Agents Chemother., 59(10):6248-55.
- 20. Reille-Seroussi, M., Gaucher, J.F., Desole, C., Gagey-Eilstein, N., Brachet, F., Broutin, I., Vidal, M., and Broussy, S. (2015). Vascular Endothelial Growth Factor peptide ligands explored by competition assay and ITC. Biochemistry, 54(33):5147-56
- 21. Monlezun L., Phan G., Benabdelhak H., Lascombe M.B., Ntsogo Enguéné V.Y., Picard M. and Broutin I. (2015). New OprM structure highlighting the nature of the N-terminal anchor. Front Microbiol. 6, 667.
- 22. Ntsogo Enguene, V.Y., Verchère, A., Phan, G., Broutin, I., and Picard, M. (2015). Catch me if you can : a biotinylated proteoliposome affinity assay for the investigation of assembly of the MexA-MexB-OprM efflux pump from Pseudomonas aeruginosa. Front Microbiol 6, 541.
- 23. Fiorini, L., Tribalat, M.A., Sauvard, L., Cazareth, J., Lalli, E., Broutin, I., Thomas, O.P., and Mus-Veteau, I. (2015). Natural paniceins from mediterranean sponge inhibit the multidrug resistance activity of patched and increase chemotherapy efficiency on melanoma cells. Oncotarget. 6(26) : 22282-97
- 24. Verchère A, Dezi M, Adrien V, Broutin I, Picard M. (2015). In vitro transport activity of the fully assembled MexAB-OprM efflux pump from Pseudomonas aeruginosa. Nat Commun., 6:6890
- 25. Richardot, C., Plesiat, P., Fournier, D., Monlezun, L., Broutin, I., and Llanes, C. (2015). Carbapenem resistance in cystic fibrosis strains of Pseudomonas aeruginosa as a result of amino acid substitutions in porin OprD. Int J Antimicrob Agents. 45(5) : 529-532
- 26. Zhang, C., Cherifi, I., Nygaard, M., Haxholm, G.W., Bogorad, R.L., Bernadet, M., England, P., Broutin, I., Kragelund, B.B., Guidotti, J.E., and Goffin, V. (2015). Residue 146 regulates prolactin receptor folding, basal activity and ligand-responsiveness : potential implications in breast tumorigenesis. Mol Cell Endocrinol 401, 173-188.
- 27. Zhang, C., Nygaard, M., Haxholm, G.W., Boutillon, F., Bernadet, M., Hoos, S., England, P., Broutin, I., Kragelund, B.B., and Goffin, V. (2015). A Residue Quartet in the Extracellular Domain of the Prolactin Receptor Selectively Controls Mitogen-Activated Protein Kinase Signaling. J Biol Chem., 290(19):11890-904.